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Am J Nucl Med Mol Imaging 2012;2(3):260-270
Original Article
[125I]FIAU imaging in a preclinical model of lung infection: quantification of
bacterial load
Mrudula Pullambhatla, Jean Tessier, Graham Beck, Bruno Jedynak, Jens U Wurthner, Martin G Pomper
Russell H. Morgan Department of Radiology and Radiological Sciences, Johns Hopkins University; AstraZeneca Discovery Medicine,
Macclesfield, U.K.; Department of Applied Mathematics and Statistics, Johns Hopkins University, Baltimore, Maryland, U.S.A.
Received May 9, 2012; accepted June 11, 2012; Epub July 10, 2012; Published July 30, 2012
Abstract: 2'-Fluoro-2'-deoxy-1β-D-arabinofuranosyl-5-[125I]iodouracil ([125I]FIAU), a substrate for the thymidine kinase (TK) present
in most bacteria, has been used as an imaging agent for single photon emission computed tomography (SPECT) in an
experimental model of lung infection. Using SPECT-CT we show that [125I]FIAU is specific for bacterial infection rather than sterile
inflammation. We report [125I]FIAU lung uptake values of 1.26 ± 0.20 percent injected dose per gram (%ID/g) in normal controls, 1.69
± 0.32 %ID/g in lung inflammation and up to 7.14 ± 1.09 %ID/g in lung infection in ex vivo biodistribution studies at 24 h after
intranasal administration of bacteria. Images of [125I]FIAU signal within lung can be used to estimate the number of bacteria
present, with a limit of detection of 109 colony forming units per mL on the X-SPECT scanner. [125I]FIAU-Based bacterial imaging
may be useful in preclinical models to facilitate the development of new antibiotics, particularly in cases where a corresponding
human trial is planned. (ajnmmi1205002)
Keywords: Inflammation, thymidine kinase, nucleoside, SPECT, PET, molecular imaging
Address all correspondence to:
Dr. Martin G Pomper
Johns Hopkins Medical Institutions
1550 Orleans Street, 492 CRB II
Baltimore, MD 21231, USA.
Tel: 410-955-2789; Fax: 443-817-0990
E-mail: mpomper@jhmi.edu
Original Article
[125I]FIAU imaging in a preclinical model of lung infection: quantification of
bacterial load
Mrudula Pullambhatla, Jean Tessier, Graham Beck, Bruno Jedynak, Jens U Wurthner, Martin G Pomper
Russell H. Morgan Department of Radiology and Radiological Sciences, Johns Hopkins University; AstraZeneca Discovery Medicine,
Macclesfield, U.K.; Department of Applied Mathematics and Statistics, Johns Hopkins University, Baltimore, Maryland, U.S.A.
Received May 9, 2012; accepted June 11, 2012; Epub July 10, 2012; Published July 30, 2012
Abstract: 2'-Fluoro-2'-deoxy-1β-D-arabinofuranosyl-5-[125I]iodouracil ([125I]FIAU), a substrate for the thymidine kinase (TK) present
in most bacteria, has been used as an imaging agent for single photon emission computed tomography (SPECT) in an
experimental model of lung infection. Using SPECT-CT we show that [125I]FIAU is specific for bacterial infection rather than sterile
inflammation. We report [125I]FIAU lung uptake values of 1.26 ± 0.20 percent injected dose per gram (%ID/g) in normal controls, 1.69
± 0.32 %ID/g in lung inflammation and up to 7.14 ± 1.09 %ID/g in lung infection in ex vivo biodistribution studies at 24 h after
intranasal administration of bacteria. Images of [125I]FIAU signal within lung can be used to estimate the number of bacteria
present, with a limit of detection of 109 colony forming units per mL on the X-SPECT scanner. [125I]FIAU-Based bacterial imaging
may be useful in preclinical models to facilitate the development of new antibiotics, particularly in cases where a corresponding
human trial is planned. (ajnmmi1205002)
Keywords: Inflammation, thymidine kinase, nucleoside, SPECT, PET, molecular imaging
Address all correspondence to:
Dr. Martin G Pomper
Johns Hopkins Medical Institutions
1550 Orleans Street, 492 CRB II
Baltimore, MD 21231, USA.
Tel: 410-955-2789; Fax: 443-817-0990
E-mail: mpomper@jhmi.edu
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