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Am J Nucl Med Mol Imaging 2012;2(2):151-162.
Original Article
Multispectral visualization of surgical safety-margins using fluorescent marker
seeds
Patrick TK Chin, Chantal AC Beekman, Tessa Buckle, Lee Josephson, Fijs WB van Leeuwen
Department of Radiology, Interventional Molecular Imaging Leiden University Medical Center (LUMC), Albinusdreef 2, 2300RC
Leiden, the Netherlands; Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School,
Building 149, 13th Street, Charlestown, MA 02129, USA. *authors contributed equally.
Received January 23, 2012; accepted February 12, 2012; Epub March 28, 2012; Published April 15, 2012
Abstract: To improve radical tumor excision during surgery, optical guidance provided by luminescent marker seeds may be suitable
for intraoperative determination of appropriate resection margins. In phantom studies we compared the tissue penetration of several
organic dyes and inorganic particles (quantum dots; QDs) after incorporation in experimental marker seeds. The tissue penetration
of (near infra-) red organic dyes was much better than the penetration of dyes and QDs with an emission in the visible range. By
combining 3 dyes in a single marker seed we were able to distinguish four depth ranges. The difference in tissue penetration
between the dyes and QDS enabled depth estimation via a ‘traffic light’ approach. (ajnmmi1201002).
Keywords: Surgical guidance, fluorescence, tissue penetration, phantom studies, marker seeds
Address all correspondence to:
Dr. van Leeuwen FWB
Department of Radiology Interventional Molecular Imaging
Leiden University Medical Center (LUMC)
Albinusdreef 2, PO Box 9600, postzone C2-S, 2300 RC
Leiden, The Netherlands.
Tel: +31(0)71 526 3491; Fax: +31(0)71 5248256
E-mail: F.W.B.van_Leeuwen@LUMC.nl
Original Article
Multispectral visualization of surgical safety-margins using fluorescent marker
seeds
Patrick TK Chin, Chantal AC Beekman, Tessa Buckle, Lee Josephson, Fijs WB van Leeuwen
Department of Radiology, Interventional Molecular Imaging Leiden University Medical Center (LUMC), Albinusdreef 2, 2300RC
Leiden, the Netherlands; Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School,
Building 149, 13th Street, Charlestown, MA 02129, USA. *authors contributed equally.
Received January 23, 2012; accepted February 12, 2012; Epub March 28, 2012; Published April 15, 2012
Abstract: To improve radical tumor excision during surgery, optical guidance provided by luminescent marker seeds may be suitable
for intraoperative determination of appropriate resection margins. In phantom studies we compared the tissue penetration of several
organic dyes and inorganic particles (quantum dots; QDs) after incorporation in experimental marker seeds. The tissue penetration
of (near infra-) red organic dyes was much better than the penetration of dyes and QDs with an emission in the visible range. By
combining 3 dyes in a single marker seed we were able to distinguish four depth ranges. The difference in tissue penetration
between the dyes and QDS enabled depth estimation via a ‘traffic light’ approach. (ajnmmi1201002).
Keywords: Surgical guidance, fluorescence, tissue penetration, phantom studies, marker seeds
Address all correspondence to:
Dr. van Leeuwen FWB
Department of Radiology Interventional Molecular Imaging
Leiden University Medical Center (LUMC)
Albinusdreef 2, PO Box 9600, postzone C2-S, 2300 RC
Leiden, The Netherlands.
Tel: +31(0)71 526 3491; Fax: +31(0)71 5248256
E-mail: F.W.B.van_Leeuwen@LUMC.nl
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